GLUTAMATE OXIDASE ENZYME ELECTRODES - MICROSENSORS FOR NEUROTRANSMITTER DETERMINATION USING ELECTROCHEMICALLY POLYMERIZED PERMSELECTIVE FILMS

Enzyme entrapment in electrochemically polymerized membranes is an att ractive method for the development of stable reproducible enzyme micro sensors with fast response times. In this paper the development of a b iosensor for the determination of glutamate, involving the entrapment of the enzyme glutamate oxidase in two different electropolymerization matrices, is described. Subsequently, the kinetics of the immobilized enzyme in the different matrices are compared, and the role of the po lymers in providing a permselective barrier for the exclusion of poten tial electroactive interferents are characterized.