HEMOSTATIC PROPERTIES OF THE SV40 TRANSFECTED HUMAN MICROVASCULAR ENDOTHELIAL-CELL LINE (HMEC-1) - A REPRESENTATIVE IN-VITRO MODEL FOR MICROVASCULAR ENDOTHELIUM

HMEC-1 is a SV-40T transfected human microvascular endothelial cell li ne that constitutively expresses RNA transcripts for plasminogen activ ator inhibitor 1 (PAI-1), tissue-type plasminogen activator (t-PA), pr otein S (PS), von Willebrand factor (vWF), and thrombomodulin. Tissue factor (TF) can be induced in response to stimulation with tumor necro sis factor alpha (TNF-alpha), interleukin-1 alpha (IL-1 alpha) and pho rbol 12-myristate 13-acetate (PMA) Proteins corresponding to PAI-1, t- PA protein S and VWF genes were constitutively released in the culture supernatant. This cell line is a model that will be useful to investi gate coagulation/fibrinolytic properties of microvascular endothelium.