APPLICATION OF DOT-IMMUNOGOLD-SILVER STAINING IN THE DIAGNOSIS OF CLONORCHIASIS

All 35 confirmed clonorchiasis cases showed a positive reaction in dot -immunogold-silver staining (Dot-IGSS) with a mean serum titre of 1:16 56, while none of the sera from 35 normal individuals reacted. A seroe pidemiological survey of middle-school students revealed a positive ra te of 17.0% (142/836), and 76.1% (105/138) of the serologically positi ve students were egg positive in stool examination. The egg positive r ates in those with antibody at levels of 1+ to 4+ were 57.1% (28/49), 75.7% (28/37), 94.7% (36/38) and 92.9% (13/14) respectively. It is bel ieved that Dot-IGSS can be used for the clinical diagnosis and epidemi ological survey of clonorchiasis.