J. Heukeshoven et R. Dernick, EFFECTIVE BLOTTING OF ULTRATHIN POLYACRYLAMIDE GELS ANCHORED TO A SOLID-MATRIX, Electrophoresis, 16(5), 1995, pp. 748-756
Ultrathin polyacrylamide gels bound on glass plates or plastic sheets
cannot be removed from their support without destruction. Therefore el
ectrophoretic transfer methods are not applicable. We have developed a
fast diffusion blotting procedure which is very simple and does not n
eed any equipment like blotting chamber or power supply. Furthermore,
no special buffer solutions are required. The method is universally ap
plicable to ultrathin sodium dodecyl sulfate, native as well as isoele
ctric focusing polyacrylamide gels.