EFFECTIVE BLOTTING OF ULTRATHIN POLYACRYLAMIDE GELS ANCHORED TO A SOLID-MATRIX

Ultrathin polyacrylamide gels bound on glass plates or plastic sheets cannot be removed from their support without destruction. Therefore el ectrophoretic transfer methods are not applicable. We have developed a fast diffusion blotting procedure which is very simple and does not n eed any equipment like blotting chamber or power supply. Furthermore, no special buffer solutions are required. The method is universally ap plicable to ultrathin sodium dodecyl sulfate, native as well as isoele ctric focusing polyacrylamide gels.