A novel strategy for identifying proteins which reveal maternal or pat
ernal inheritance in the mouse is presented. Using two-dimensional ele
ctrophoresis we investigated protein expression patterns of adult live
r and different embryonic and extraembryonic tissue in C57BL/6Crl and
in DBA/2Crl mice, as well as in their reciprocal hybrids. We found thr
ee groups of protein spots showed maternal or paternal inheritance of
quantitative variations. proteins were characterized by N-terminal or
internal amino acid sequencing, by determination of the amino acid com
position, by glycoprotein staining and RNA expression analysis. The th
ree proteins identified were: alpha-enolase, cyclophilin and beta-grou
p hemoglobins. The parental effects observed for alpha-enolase and cyc
lophilin were found to be due to parent-specific post-translational mo
difications of these proteins. For the beta-group hemoglobins our resu
lts suggested parental effects on the transcriptional level.