DETERMINATION OF ACEFYLLINE HEPTAMINOL IN PHARMACEUTICAL PREPARATIONSBY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

This paper describes two simple and rapid procedures for the determina tion of heptaminol and of both acetylline and benzyl alcohol, using re versed phase high performance liquid chromatography under isocratic co nditions and UV spectrophotometric detection. For heptaminol, procedur e includes a precolumn derivatizatin by orthophthaldialdehyde in prese nce of reducing agent, in alkaline buffer. It is specific, linear, acc urate (recovery mean = 99.90 +/- 0.44%), reliable (precision = 1.00%) and sensitive (0.007 mg/l in the drug). For acefylline and benzyl alco hol, the procedure uses hexadecyltrimethylammonium bromide, for an ion -pair technique. It is specific, linear, accurate (recovery means = 99 .65 +/- 0.52% and 99.65 +/- 1.10% respectively), reliable (precisions < 1.5%) and sensitive (0.30 mg/l and 0.01 mu l/l respectively, in the drug).