PULMONARY TOXOPLASMOSIS IN HIV-INFECTED PATIENTS - USEFULNESS OF POLYMERASE CHAIN-REACTION AND CELL-CULTURE

Toxoplasmosis is a serious opportunistic infection in patients with ac quired immunodeficiency syndrome (AIDS), The lung is a major site of i nfection after the central nervous system. The aim of the study was to assess the polymerase chain reaction (PCR) and cell culture for the d etection of Toxoplasma gondii. One hundred and thirty two human immuno deficiency virus (HIV)-infected patients with respiratory manifestatio ns, who underwent fibreoptic bronchoalveolar lavage, were investigated , Detection of Toxoplasma gondii was compared using three techniques: Giemsa staining; polymerase chain reaction with specific primers deriv ed from the P30 gene; and culture on the MRC5 cell line. Toxoplasma go ndii was detected in the same four samples by all three techniques. We conclude that PCR adds little to conventional (and cheaper) tools alr eady used to diagnose pulmonary toxoplasmosis.