CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A LIPASE FROM STAPHYLOCOCCUS-HYICUS

Authors
RANSAC S BLAAUW M DIJKSTRA BW SLOTBOOM AJ BOOTS JWP VERHEIJ HM
Citation
S. Ransac et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF A LIPASE FROM STAPHYLOCOCCUS-HYICUS, Journal of structural biology, 114(2), 1995, pp. 153-155
Citations number
20
Categorie Soggetti
Cell Biology",Biology
Journal title
Journal of structural biology → ACNP
ISSN journal
10478477
Volume
114
Issue
2
Year of publication
1995
Pages
153 - 155
Database
ISI
SICI code
1047-8477(1995)114:2<153:CAPAOA>2.0.ZU;2-W
Abstract
Single crystals of a lipase from Staphylococcus hyicus have been obtai ned using a combination of 18 to 24% dimethylsulfoxide and 10% isoprop anol as a precipitant. The crystals grow at 4 degrees C in 2-3 months. They belong to the orthorhombic space group P2(1)2(1)2(1) with a = 73 .31 Angstrom, b = 77.96 Angstrom, and c = 169.81 Angstrom, with two pr otein molecules per asymmetrical unit. The crystals diffract to at lea st 2.8 Angstrom resolution and are suitable for an X-ray structure ana lysis. (C) 1995 Academic Press,lnc.