R. Cachon et al., KINETIC-BEHAVIOR OF LACTOCOCCUS-LACTIS SSP LACTIS BV DIACETYLACTIS IMMOBILIZED IN CALCIUM ALGINATE GEL BEADS, Process biochemistry, 30(6), 1995, pp. 503-510
Alginate beads with entrapped Lactococcus lactis spp. lactis by. diace
tylactis were used as biocatalysts in continuous fermentation and the
dynamics of the system analysed. Colonization by cells changed during
fermentation, concentrating at the periphery (where cell densities rea
ch 350 g litre(-1)). In the steady state, colonization of the gel was
interrupted while growth continued, producing cells that were released
into the effluent medium. The initial cell concentration in the beads
had little influence on steady-state gel activity, while bead radius
(0.75 to 1.5 mm) modified growth and acid production. A higher growth
yield and cell release rate were obtained with smaller beads. Activity
inside beads was not limited by external transfer, but rather by inte
rnal transfer and by the reaction. The increase of citrate concentrati
on in the feeding medium activates citrate utilization, diacetyl + ace
toin production, and leads to a great enhancement of citrate bioconver
sion to aroma compounds.