Furosine, an index of the initial steps of Maillard reaction, has been
used for the evaluation of shelf life in UHT milk and of heat damage
in various food products. The aim of this paper was to study furosine
as an index for egg freshness. Fresh eggs from different hen breeds we
re used to assess HPLC method repeatability, to investigate natural va
riability of furosine content in fresh shell eggs, and to study furosi
ne formation during storage at 5, 20, 30, and 38 degrees C. Furosine,
present in fresh egg albumen at a level of about 10 mg/100 g of protei
n, increased during storage with dependence on temperature, reaching a
fter 40 days at 20 degrees C a level of about 100 mg/100 g of protein.
The modification of furosine content in yolk during storage at 20 deg
rees C was minimal. Furosine, when measured in albumen, resulted in a
very reliable index for egg freshness. In albumen, maximum furosine co
ntents of 60 and 90 mg/100 g of protein, respectively, for grades A-ex
tra and A eggs commercialized in the European Community are suggested.