PRODUCTION OF MONOCLONAL-ANTIBODIES FOR THE SPECIFIC DETECTION OF DEOXYNIVALENOL AND 15-ACETYLDEOXYNIVALENOL BY ELISA

Monoclonal antibodies (MABs) against the mycotoxin deoxynivalenol (DON ) were produced using an immunogen consisting of DON conjugated to bov ine serum albumin (BSA) through its 15-hydroxyl group (15-DON-BSA). Fr om 80 hybridomas, the four best stabilized hybridoma cell lines secret ing anti-DON MABs of subclass IgG(1) were used to prepare ascites in m ice. The MABs purified from ascites were used to detect DON by direct competitive ELISA. The MABs were very specific to 15-acetyl-DON and DO N but had negligible binding to 3-acetyl-DON, T-2 toxin, sambucinol, a nd neosolaniol. The effective range of detection was 0.05-20 mu g/mL o f DON or 15-acetyl-DON. These antibodies also showed a high stability toward grain extracts containing up to 40% methanol.