DEVELOPMENT AND VALIDATION OF A MONOCLONAL ANTIBODY-BASED ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR SALINOMYCIN IN CHICKEN LIVER-TISSUE

Salinomycin is one of the most widely used coccidiostats in U.S. agric ulture. A rapid and accurate analytical method for this drug should pr ovide producers and users with an effective management tool. The curre nt chromatographic methods are sensitive but are labor-intensive. In a ddition, they require large amounts of expensive organic solvents for extraction and cleanup, which requires proper (and expensive) disposal . This paper reports the development of an enzyme-linked immunosorbent assay (ELISA) coupled to a simple aqueous extraction procedure for th e analysis of salinomycin in chicken liver tissue. Recovery from spike d liver homogenates was quantitative in the range from 5.0 to 0.05 ppm . Analysis of chicken livers containing incurred residue by ELISA and highperformance liquid chromatography (HPLC) showed the results to be highly correlated (p < 0.0001). The ELISA method described here has a limit of quantitation of 50 ppb, which is more sensitive than the HPLC method.