EFFECTS OF BRADYKININ AND BRADYKININ ANALOGS ON SPLEEN-CELLS OF MICE

The present study was undertaken to examine the effects of bradykinin and selected bradykinin analogues on mononuclear cells derived from mo use spleen. Bradykinin as well as des-Arg(9)-bradykinin, a bradykinin B-1 receptor agonist, were able to induce the release of so-called cha rge-changing lymphokines, which could be identified as interleukin-1, interleukin-6, interleukin-2 and as interleukin-2 receptor. The cytoki ne release evoked by bradykinin and all analogues showed a bell-shaped dose dependence in a range of 10(-8) M to 10(-6) M and could be inhib ited by the specific bradykinin receptor antagonist, D-Arg(0)[Hyp(3),T hi(5),D-Tic(7),Oic(8)]bradykinin (HOE140), and by bradykinin analogues with N-methyl-phenylalanine at position 2 in concentrations as low as 10(-12) M and 10(-13) M, respectively. Obviously the N-terminus of br adykinin seems to be responsible for the interaction with the mononucl ear cells concerning all peptides investigated.