DIRECT AND COMPETITIVE KINETIC-ANALYSIS OF THE INTERACTION BETWEEN HUMAN IGG1 AND A ONE DOMAIN ANALOG OF PROTEIN-A

Direct and competitive kinetic analysis of the binding between a one d omain analogue of protein A, and mutants thereof, to immobilised hIgG1 was compared using a biosensor system based on surface plasmon resona nce detection. Rate constants determined from both assays were almost identical. The experiments demonstrate that competitive kinetic analys is can be used in combination with biosensor technology, and indicate that competitive kinetic analysis may extend the use of the technology to include low molecular weight analytes.