PYROLYSIS GAS-CHROMATOGRAPHY MASS-SPECTROMETRY CHARACTERIZATION OF WHEAT-STRAW ALKALINE-COOKING EFFLUENTS AFTER BIOLOGICAL TREATMENT WITH THE FUNGI PHANEROCHAETE-CHRYSOSPORIUM AND GANODERMA-AUSTRALE
Am. Calvo et al., PYROLYSIS GAS-CHROMATOGRAPHY MASS-SPECTROMETRY CHARACTERIZATION OF WHEAT-STRAW ALKALINE-COOKING EFFLUENTS AFTER BIOLOGICAL TREATMENT WITH THE FUNGI PHANEROCHAETE-CHRYSOSPORIUM AND GANODERMA-AUSTRALE, Analytica chimica acta, 309(1-3), 1995, pp. 145-152
Pyrolysis-gas chromatography-mass spectrometry (PB-GC-MS) was applied
in order to monitor the extent of chemical modification of lignin unit
s after seven-day treatment of paper-industry wastewaters using two li
gninolytic fungi. Py-GC-MS after the effluent treatment with Ganoderma
australe revealed an increase in the lignin compounds, in which oxida
tion and shortening of the side chain of lignin molecules have occurre
d. In addition, no significant alkalilignin weight-loss nor reduction
of lignin pyrolysis products were detected. On the other hand, these c
hemical transformations of lignin units were not observed after the ef
fluent treatment with Phanerochaete chrysosporium. However, a remarkab
le reduction of lignin pyrolysis products and a large alkalilignin wei
ght-loss were observed. The percentages of decolorization attained at
day seven of culture were similar for both fungi. These results are po
ssibly due to the different lignin-degrading mechanisms and/or to the
earlier expression of the ligninolytic system of P. chrysosporium, und
er the experimental conditions assayed. Effluent decolorization could
not be attributed to adsorption on the mycelia, since no significant q
uantity of alkalilignin was found attached to the mycelia of either fu
ngi.