ENZYMATIC-SYNTHESIS OF MANNOBIOSES AND MANNOTRIOSES BY REVERSE HYDROLYSIS USING ALPHA-MANNOSIDASE FROM ASPERGILLUS-NIGER

Authors
AJISAKA K MATSUO I ISOMURA M FUJIMOTO H SHIRAKABE M OKAWA M
Citation
K. Ajisaka et al., ENZYMATIC-SYNTHESIS OF MANNOBIOSES AND MANNOTRIOSES BY REVERSE HYDROLYSIS USING ALPHA-MANNOSIDASE FROM ASPERGILLUS-NIGER, Carbohydrate research, 270(2), 1995, pp. 123-130
Citations number
18
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
270
Issue
2
Year of publication
1995
Pages
123 - 130
Database
ISI
SICI code
0008-6215(1995)270:2<123:EOMAMB>2.0.ZU;2-I
Abstract
Various manno-oligosaccharides including alpha-D-man-(1-->2)-D-man and alpha-D-man-(1-->2)-alpha-D-man-(1-->2)-D-man were formed when a high ly concentrated mannose solution was incubated in the presence of alph a-mannosidase from Aspergillus niger. alpha-D-Man-(1-->2)-D-man and al pha-D-man-(1-->2)-alpha-D-man-(1-->2)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatogra phy using an amino-silica column. In addition to the above oligosaccha rides, alpha-D-man-(1-->3)-D-man, alpha-D-man-(1 -->6)-D-man, and alph a-D-man-(1-->2)-alpha-D-man-(1-->6)-D-man were also isolated.