ONE-STEP ELECTROBLOTTING OF 2- TO 100-KDA PROTEINS ONTO A PVDF MEMBRANE

Authors
PARRADO J AYALA A MACHADO A
Citation
J. Parrado et al., ONE-STEP ELECTROBLOTTING OF 2- TO 100-KDA PROTEINS ONTO A PVDF MEMBRANE, Applied biochemistry and biotechnology, 53(3), 1995, pp. 285-292
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
53
Issue
3
Year of publication
1995
Pages
285 - 292
Database
ISI
SICI code
0273-2289(1995)53:3<285:OEO2T1>2.0.ZU;2-T
Abstract
This article describes a method for electroblotting peptides and small proteins (<100 kDa) from tricine gels onto a PVDF membrane. The major potential problem with these types of procedures is that proteins ten d to stay in the gel under conditions where peptides are effectively e luted. The suggested protocol allows the complete transfer and binding of proteins and peptides in the range of 2-97 kDa.