Three series of data obtained in a pilot-scale alternating-type activa
ted sludge nutrient removal process are examined in light of the recen
tly introduced Activated Sludge Model No.2 (ASM2). The first data seri
es was collected while adding acetate to the inlet to improve phosphat
e removal. ASM2 can explain why phosphate levels first rise before an
improvement occurs based on a slow build-up of internally stored polyh
ydroxyalkanoates (PHA). The second and third data series represent bat
ch experiments performed in the pilot plant, and show the effect of ni
trate on phosphate release. ASM2 could describe the second data series
well after including one additional reaction to account for denitrifi
cation by phosphate accumulating organisms. The third data series exam
ines the effect of the rate of acetate addition, and shows that this a
ffects the rate of anoxic phosphate release more strongly than it does
the denitrification rate.