CHEMICAL SYNTHESIS OF A GENE FOR THE PROT EINASE-INHIBITOR EGLIN-C WITHOUT USING T4 DNA-LIGASE AND ITS EXPRESSION IN ESCHERICHIA-COLI

Authors
VEIKO VP OSIPOV AS SHEKHTER II BULENKOV MT RATMANOVA KI GULKO LB CHIBISKOVA NA ERREIS LL DEREVSHCHIKOVA EB DEBABOV VG
Citation
Vp. Veiko et al., CHEMICAL SYNTHESIS OF A GENE FOR THE PROT EINASE-INHIBITOR EGLIN-C WITHOUT USING T4 DNA-LIGASE AND ITS EXPRESSION IN ESCHERICHIA-COLI, Bioorganiceskaa himia, 21(5), 1995, pp. 354-358
Citations number
18
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
21
Issue
5
Year of publication
1995
Pages
354 - 358
Database
ISI
SICI code
0132-3423(1995)21:5<354:CSOAGF>2.0.ZU;2-U
Abstract
A synthetic gene for a proteinase inhibitor (eglin C) that was obtaine d by direct amplification with oligonucleotides without using DNA liga se and polynucleotide kinase of T4 phage was cloned into expression ve ctors. A high yield of the polypeptide (110 - 130 mg/l) was attained i n E. coli strains.