Nv. Doronina et al., ISOLATION AND CHARACTERIZATION OF A NEW FACULTATIVELY METHYLOTROPHIC BACTERIUM - DESCRIPTION OF METHYLORHABDUS-MULTIVORANS, GEN-NOV, SP-NOV, Systematic and applied microbiology, 18(1), 1995, pp. 92-98
A new Gram-negative aerobic, asporogenous, nonmotile, colourless, facu
ltatively methylotrophic bacterium with the serine pathway of C1 assim
ilation is described. The organism (strain DM13) is a short rod multip
lying by fission with the formation of a constriction connecting fresh
ly divided cells. The G+C content of DNA is 66,2 mol %. The major cell
ular fatty acids are cis-vaccenic, lactobacillic and palmitic acid. Th
e major ubiquinone is Q-10. The bacterium utilizes dichloromethane, me
thanol and methylamine as C1 sources of carbon and energy as well as a
wide range of polycarbon compounds. It shows a low degree of DNA-DNA
similarity (<10%) with representatives of the genera Pseudomonas, Meth
ylobacterium, Methylomicrobium, Hyphomicrobium, and Aminobacter. Based
on morphological, pheno- and genotypic distinctions, the isolate is p
roposed to represent a new genus - Methylorhabdus with the type strain
Methylorhabdus multivorans gen. nov., sp. nov. The characteristics of
the new genus and species are presented.