DIVERSITY AND GENETIC RELATEDNESS WITHIN GENERA XANTHOMONAS AND STENOTROPHOMONAS USING RESTRICTION-ENDONUCLEASE SITE DIFFERENCES OF PCR-AMPLIFIED 16S RIBOSOMAL-RNA GENE
X. Nesme et al., DIVERSITY AND GENETIC RELATEDNESS WITHIN GENERA XANTHOMONAS AND STENOTROPHOMONAS USING RESTRICTION-ENDONUCLEASE SITE DIFFERENCES OF PCR-AMPLIFIED 16S RIBOSOMAL-RNA GENE, Systematic and applied microbiology, 18(1), 1995, pp. 127-135
Restriction maps of PCR-amplified 16s rRNA genes (rrs for ribosomal rR
NA small subunit) were prepared and compared for 43 Xanthomonas and St
enotrophomonas strains belonging to representative DNA-DNA similarity
groups. Nineteen restriction fragment length polymorphisms (RFLPs) wer
e distinguished, forming two main clusters: one contained all Xanthomo
nas species, the other contained strains of Stenotrophomonas spp. (for
merly named X. maltophilia). The genera Xanthomonas and Stenotrophomon
as appeared to form a coherent group excluding Xylella fastidiosa. The
rrs RFLP permitted to differentiate X. fragariae, X. oryzae, X. axono
podis, X. populi pv. populi from most pathovars of X. campestris. The
latter species had very closely related patterns, contrary to X. albil
ineans, X. campestris pv. hyacinthi and pv. graminis, and a X. campest
ris strain (isolated from Saccharum officinarum, Guadeloupe) whic form
ed a more distinct subcluster. All genospecies of Stenotrophomonas had
different rrs patterns, and appeared to form a less coherent taxon. R
elationships between the percentage of DNA-DNA hybridization and the r
ate of nucleotide substitution in rrs confirmed the clear delineation
of the two genera.