OVERPRODUCTION OF HIS-TAGGED ECORII RESTRICTION-ENDONUCLEASE AND TERMINALLY DELETED MUTANT PROTEINS

Authors
KUPPER D REUTER M KRUGER DH
Citation
D. Kupper et al., OVERPRODUCTION OF HIS-TAGGED ECORII RESTRICTION-ENDONUCLEASE AND TERMINALLY DELETED MUTANT PROTEINS, Gene, 157(1-2), 1995, pp. 97-98
Citations number
3
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
157
Issue
1-2
Year of publication
1995
Pages
97 - 98
Database
ISI
SICI code
0378-1119(1995)157:1-2<97:OOHERA>2.0.ZU;2-3
Abstract
EcoRII was the first restriction endonuclease (ENase) reported requiri ng the cooperative interaction with at least two DNA sites for activit y. Using two different expression systems the enzyme could be purified and its special substrate requirements were further analyzed. At the present state of knowledge we suggest a model of simultaneous binding of two DNA sites to one dimeric enzyme molecule (see Kruger et al., FE MS Microbiol. Rev. (1995) in press).