PCR-DIRECTED PREPARATION AND SINGLE-STEP PURIFICATION OF HIGHLY-ACTIVE HISTIDINE-TAGGED RESTRICTION-ENDONUCLEASE HGIBI (GGWCC)

The polymerase chain reaction was used to produce His, fusion proteins via deletion of an intervening piece of DNA. The generally applicable method was performed using a standard primer with the advantage that the fusion does not produce additional amino acids. In a single-step p urification highly purified, enzymatically active restriction endonucl ease was obtained.