MUTATIONAL ANALYSIS OF CONSERVED AMINO-ACID MOTIFS IN ECOKI ADENINE METHYLTRANSFERASE

Authors
DRYDEN DTF WILLCOCK DF MURRAY NE
Citation
Dtf. Dryden et al., MUTATIONAL ANALYSIS OF CONSERVED AMINO-ACID MOTIFS IN ECOKI ADENINE METHYLTRANSFERASE, Gene, 157(1-2), 1995, pp. 123-124
Citations number
6
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
157
Issue
1-2
Year of publication
1995
Pages
123 - 124
Database
ISI
SICI code
0378-1119(1995)157:1-2<123:MAOCAM>2.0.ZU;2-Y
Abstract
The EcoKI methyltransferase (M . EcoKI, MTase) contains the amino acid (aa) sequences AAGTA and NPPF believed to represent the two sequences that are strongly conserved in adenine MTases [Klimasauskas et al., N ucleic Acids Res. 17 (1989) 9823-9831]. We have analysed a mutation in the first sequence that abolishes cofactor binding and enzyme activit y, and mutations in the second sequence that reduce or abolish activit y without affecting cofactor and DNA binding.