DISRUPTION OF THE TARGET G-C BASE-PAIR BY THE HHAI METHYLTRANSFERASE

Authors
KLIMASAUSKAS S ROBERTS RJ
Citation
S. Klimasauskas et Rj. Roberts, DISRUPTION OF THE TARGET G-C BASE-PAIR BY THE HHAI METHYLTRANSFERASE, Gene, 157(1-2), 1995, pp. 163-164
Citations number
7
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
157
Issue
1-2
Year of publication
1995
Pages
163 - 164
Database
ISI
SICI code
0378-1119(1995)157:1-2<163:DOTTGB>2.0.ZU;2-Y
Abstract
HhaI MTase binds DNA duplexes containing mismatches at the target base position with higher affinity than that observed for the canonical su bstrate. The stability of these MTase-DNA complexes inversely correlat es with the strength of the base pair that is disrupted upon interacti on. This finding may offer a general tool to detect other enzymes that hip bases out of the DNA helix.