EFFECT OF 5-AZACYTIDINE AND SINEFUNGIN ON STREPTOMYCES DEVELOPMENT

The effect of two DNA-methyltransferase inhibitors, 5-azacytidine (5az aC) and sinefungin (Sf), on the development of Streptomyces antibiotic us ETH7451 (Sa) was studied. Pulse labeling experiments and SDS-PAGE a nalysis of proteins from cells grown in sporulation synthetic medium s howed that both inhibitors affect a limited number of systems. Synthes is of the antibiotic rhodomycin was increased in the presence of 5azaC . 5azaC also stimulated the production of actinorhodin in cultures of S. coelicolor A3(2) grown in minimal medium. The analog did not affect the expression of whiB and whiG, two sporulation genes from S. coelic olor A3(2) whose homologues are present in Sa. Overall results indicat ed that 5azaC and Sf affect specific events associated with differenti ation and secondary metabolism in Streptomyces.