MECHANISM OF ACTIVE DNA DEMETHYLATION DURING EMBRYONIC-DEVELOPMENT AND CELLULAR-DIFFERENTIATION IN VERTEBRATES

Authors
JOST JP JOST YC
Citation
Jp. Jost et Yc. Jost, MECHANISM OF ACTIVE DNA DEMETHYLATION DURING EMBRYONIC-DEVELOPMENT AND CELLULAR-DIFFERENTIATION IN VERTEBRATES, Gene, 157(1-2), 1995, pp. 265-266
Citations number
15
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
157
Issue
1-2
Year of publication
1995
Pages
265 - 266
Database
ISI
SICI code
0378-1119(1995)157:1-2<265:MOADDD>2.0.ZU;2-C
Abstract
Incubation of hemimethylated and labelled oligodeoxynucleotides with n uclear extracts from differentiating chicken embryos and mouse myoblas ts resulted in the replacement of m(5)C by C. One of the enzymes invol ved is m(5)CpG endonuclease. It cleaves only m(5)CpG and not, m(5)CpT, m(5)CpA, m(5)CpC or m(6)ApT. The enzyme is not sequence specific and catalyses the reaction in the presence of high concentrations of EDTA or EGTA.