HYPOXIA AND REOXYGENATION-INDUCED INJURY OF RENAL EPITHELIAL-CELLS - EFFECT OF FREE-RADICAL SCAVENGERS

The aim of this study was to characterize injuries of LLC-PK1 and MDCK cells exposed to hypoxia and reoxygenation. Exposure of LLC-PK, cells to hypoxia reduced the ATP contents and increased the leakage of lact ate dehydrogenase (LDH), but MDCK cells had no such injuries. Hypoxia- reoxygenation of LLC-PK1 cells dramatically increased LDH leakage, whi ch was suppressed by free radical scavengers, N,N'-diphenyl-p-phenylen ediamine, superoxide dismutase and N,N'-dimethylthiourea. These result s suggest that use of LLC-PK1 cells has advantages for the investigati on of ischemia-reperfusion injury of the kidney as an in vitro model a nd that generation of oxygen radicals is involved in the cellular inju ry induced by hypoxia-reoxygenation.