A NEW CELL-LINE FROM HUMAN UNDIFFERENTIATED CARCINOMA OF THE OVARY - ESTABLISHMENT AND CHARACTERIZATION

A cell line designated CUMO-2 has been established from an undifferent iated ovarian carcinoma. The s.c. injection of cells into nude mice ga ve rise to fast-growing tumors, while the i.p. route induced a periton eal carcinomatosis with ascites. Histopathologically, the transplanted s.c. tumors closely resembled the original tumor, but tumors develope d in the peritoneal cavity were highly anaplastic. The epithelial natu re of the cells was confirmed by ultrastructural analysis. Sequential cytogenetic analyses on early and late passages revealed highly aneupl oid tumor cells with consistent structural aberrations of chromosomes 1, 3, 8 and 11. CUMO-2 cells were found to produce CA 125 in vitro and in vivo. Cytosol estrogen receptor (ER) was found but progesterone re ceptor (PR) was not measured. HLA typing indicated the presence of DR8 and DQw4. A gonadotropin-releasing hormone (Gn-RH) analog inhibited c ell growth and Gn-RH receptor mRNA was detected by reverse transcripti on/polymerase chain reaction in this cell line. Administration of tran sforming growth factor beta 1 inhibited both cell growth and c-myc mRN A expression. This cell line demonstrated a conformational band shift in exon 7 of the p53 gene. It was a frameshift mutation.