PREDICTION AND FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ESTIMATION OF THE SECONDARY STRUCTURE OF A RECOMBINANT BETA-GLUCOSIDASE FROM STREPTOMYCES SP (ATCC-11238)
Ja. Perezpons et al., PREDICTION AND FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ESTIMATION OF THE SECONDARY STRUCTURE OF A RECOMBINANT BETA-GLUCOSIDASE FROM STREPTOMYCES SP (ATCC-11238), Biochemical journal, 308, 1995, pp. 791-794
The secondary structure of a recombinant beta-glucosidase (EC 3.2.1.21
) from Streptomyces sp. (ATCC 11238) has been predicted by computer al
gorithms and also estimated by Fourier-transform IR spectroscopy. From
curve fitting of the deconvoluted IR spectra, the most probable distr
ibution of the secondary-structural classes appears to be about 34% al
pha-helix, 30% beta-sheet, 25% reverse turns and 11% non-ordered struc
tures. These data showed a good agreement with data from computer pred
iction (35% alpha-helix, 23% beta-sheet, 31% reverse turns and 11% non
-ordered structures).