PREDICTION AND FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ESTIMATION OF THE SECONDARY STRUCTURE OF A RECOMBINANT BETA-GLUCOSIDASE FROM STREPTOMYCES SP (ATCC-11238)

Authors
PEREZPONS JA PADROS E QUEROL E
Citation
Ja. Perezpons et al., PREDICTION AND FOURIER-TRANSFORM INFRARED-SPECTROSCOPY ESTIMATION OF THE SECONDARY STRUCTURE OF A RECOMBINANT BETA-GLUCOSIDASE FROM STREPTOMYCES SP (ATCC-11238), Biochemical journal, 308, 1995, pp. 791-794
Citations number
46
Categorie Soggetti
Biology
Journal title
Biochemical journal → ACNP
ISSN journal
02646021
Volume
308
Year of publication
1995
Part
3
Pages
791 - 794
Database
ISI
SICI code
0264-6021(1995)308:<791:PAFIEO>2.0.ZU;2-H
Abstract
The secondary structure of a recombinant beta-glucosidase (EC 3.2.1.21 ) from Streptomyces sp. (ATCC 11238) has been predicted by computer al gorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distr ibution of the secondary-structural classes appears to be about 34% al pha-helix, 30% beta-sheet, 25% reverse turns and 11% non-ordered struc tures. These data showed a good agreement with data from computer pred iction (35% alpha-helix, 23% beta-sheet, 31% reverse turns and 11% non -ordered structures).