P. Viani et al., EFFECT OF ENDOTHELIN-1 INDUCED ISCHEMIA ON PEROXIDATIVE DAMAGE AND MEMBRANE-PROPERTIES IN RAT STRIATUM SYNAPTOSOMES, Neurochemical research, 20(6), 1995, pp. 689-695
Synaptosomes obtained from rat striata lesioned by central injection o
f endothelin-1 (ET-1) were analyzed for the levels of lipid peroxidati
on products, the susceptibility to lipid peroxidation, the phospholipi
d and free fatty acid composition and the activity of Na+,K+-ATPase on
e hour after ET-1 treatment. The intrastriatal injection of ET-1 promo
ted an increase of endogenous thiobarbituric reactive substances (TEAR
S), as index of free radical mediated lipid damage, and a greater susc
eptibility to iron/ascorbate-induced lipid peroxidation. The pattern o
f free fatty acids showed a significant decrease of arachidonic and do
cosahexaenoic acid consequent to ET-1 treatment. The analysis of lipid
composition showed a significant loss of phospholipids: among phospho
lipid species, sphingomyelin and phosphatidylethanolamine plasmalogen
were particularly reduced by ET-1 treatment. The activity of membrane-
bound Na+,K+-ATPase was also significantly reduced in synaptosomes obt
ained from ET-1 lesioned striata. Taken together these results indicat
e a significant modification of synaptosomal membrane of ET-1 treated
rat striata, possibly due to a free radical mediated damage.