IDENTIFICATION AND RELATIONSHIPS OF CULTIVATED ACCESSIONS FROM LOLIUM-FESTUCA COMPLEX BASED ON RAPD FINGERPRINTING

Randomly Amplified Polymorphic DNA (RAPD) technique with 15 arbitrary primers was used to identify and reveal relationships of the accession s comprising 4 species of Lolium-Festuca complex. Altogether 252 RAPD markers were considered in statistical treatment, 60 of which could be identified as potentially taxon-specific. All analyzed taxa were full y distinguishable using RAPD markers. Lolium-Festuca relationships bas ed on RAPD data were evaluated using cluster analysis (UPGMA) and prin ciple coordinate analysis (PCO). The results of UPGMA as well as PCO p erformed on data pooled from all RAPD profiles support separation of t he two genera Lolium and Festuca.