THE PTUGA AND PTUGAS VECTORS FOR HIGH-LEVEL EXPRESSION OF CLONED GENES IN ESCHERICHIA-COLI

Plasmids pTugA and pTugAS, designed for expression of cloned genes in Escherichia coli, possess the features of high-level inducible transcr iption, enhanced RNA translation, portability, high copy number, stabi lity and versatility. In addition, pTugAS can be used to produce fusio n proteins comprising a target protein and a cellulose-binding domain. Such fusion proteins can be purified in a single step by affinity chr omatography on cellulose. Expression of two model gene fusions using t he pTug plasmids resulted in yields of 500 mg of intracellular and 250 mg of extracellular recombinant protein per liter.