THE DIHYDROFOLATE REDUCTASE-ENCODING GENE DYRA OF THE HYPERTHERMOPHILIC BACTERIUM THERMOTOGA-MARITIMA

Authors
VANDECASTEELE M LEGRAIN C WILQUET V GLANSDORFF N
Citation
M. Vandecasteele et al., THE DIHYDROFOLATE REDUCTASE-ENCODING GENE DYRA OF THE HYPERTHERMOPHILIC BACTERIUM THERMOTOGA-MARITIMA, Gene, 158(1), 1995, pp. 101-105
Citations number
20
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
158
Issue
1
Year of publication
1995
Pages
101 - 105
Database
ISI
SICI code
0378-1119(1995)158:1<101:TDRGDO>2.0.ZU;2-0
Abstract
The structural gene (dyrA) encoding dihydrofolate reductase (DHFR) of Thermotoga maritima has been cloned, sequenced and expressed in Escher ichia coli. The dyrA gene, located immediately upstream from the gene encoding aspartate carbamoyltransferase (pyr B), encodes a highly ther mostable enzyme with a distinct thermophilic activity profile. Importa nt structural features are conserved among all bacterial DHFR, yet the DHFR of T. maritima appears unique in a number of insertions and dele tions, some of which are reminiscent of eukaryotic DHFR.