EXPRESSION AND REGULATION OF THE NEUROPEPTIDE-Y Y2 RECEPTOR IN SENSORY AND AUTONOMIC GANGLIA

Citation
X. Zhang et al., EXPRESSION AND REGULATION OF THE NEUROPEPTIDE-Y Y2 RECEPTOR IN SENSORY AND AUTONOMIC GANGLIA, Proceedings of the National Academy of Sciences of the United Statesof America, 94(2), 1997, pp. 729-734
Citations number
38
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
94
Issue
2
Year of publication
1997
Pages
729 - 734
Database
ISI
SICI code
0027-8424(1997)94:2<729:EAROTN>2.0.ZU;2-K
Abstract
The Y2 subtype of neuropeptide tyrosine (NPY) receptors (Y2R) and some neuropeptides have been studied with in situ hybridization in sensory and autonomic neurons of rat and monkey. Between 10% and 20% of the l umbar dorsal root ganglion (DRG) neuron profiles (NPs) contain Y2R mRN A in the rat and monkey. In rat DRGs Y2R mRNA is expressed in calciton in gene-related peptide (CGRP)-positive, medium-sized, and large neuro ns, that is in a complementary fashion to the Y1R that is located in s mall CGRP neurons. In monkey DRGs Y2R mRNA is expressed mainly in smal l neurons. Peripheral axotomy up-regulates the Y2R in small and large DRG neurons in both species. Y2R and NPY mRNAs are colocalized in many large neurons in axotomized rat DRGs. Y2R mRNA is expressed in 50% of the NPs in the nodose gang-lion with a modest increase after axotomy. Y2R mRNA is detected in a few NPs in normal rat superior cervical gan glia, with a marked increase after transection of the carotid nerves. No Y2R mRNA-positive, but many (approximate to 30%) weakly Y1R mRNA-po sitive NPs were found in the sphenopalatine ganglion. Finally, Y2R mRN A levels increase in rat spinal motoneurons after axotomy. Thus, under normal circumstances NPY may act on Y1 and Y2Rs expressed, respective ly, in small and large CGRP-positive DRG neurons in the rat. Y2R may b e an important receptor in the viscero-sensory neurons. Y2Rs may be pa rticularly important after axotomy serving as presynaptic and/or autor eceptors on rat DRG, superior cervical ganglion, and nodose ganglion n eurons and as presynaptic receptors in monkey DRG neurons.