GENOMIC DIVERSITY OF FIELD ISOLATES OF BURKHOLDERIA-SOLANACEARUM IN JAPAN

Genomic differences between 18 isolates of Burkholderia solanacearum o riginated from diseased plants cultivated in nine different agricultur al fields were examined by using four DNA-based methods: repetitive DN A polymorphism analysis, restriction-site analysis of polymerase chain reaction products, random amplified polymorphic DNA (RAPD) analysis, and macrorestriction analysis. Genomic diversity among the isolates wa s detected by RAPD and macrorestriction analysis. The latter revealed the regional variation of the genome structure among the isolates, sug gesting that B. solanacearum populations consist of a number of indepe ndent clonal lines.