LOCALIZATION OF INTEGRIN SUBUNITS ALPHA-6 AND BETA-1 DURING SOMITOGENESIS IN THE LONG-TAILED MACAQUE (MACACA-FASCICULARIS)

Citation
Cst. Pow et Ag. Hendrickx, LOCALIZATION OF INTEGRIN SUBUNITS ALPHA-6 AND BETA-1 DURING SOMITOGENESIS IN THE LONG-TAILED MACAQUE (MACACA-FASCICULARIS), Cell and tissue research, 281(1), 1995, pp. 101-108
Citations number
54
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0302766X
Volume
281
Issue
1
Year of publication
1995
Pages
101 - 108
Database
ISI
SICI code
0302-766X(1995)281:1<101:LOISAA>2.0.ZU;2-U
Abstract
The distribution of integrin subunits alpha 6 and beta 1, and the alph a 6 beta 1 integrin ligand, laminin, was examined during somitogenesis in developmental stages 11, 13, and 16 in the long-tailed macaque, us ing peroxidase immunocytochemistry. Within differentiating somites in stage 11, alpha 6 expression was observed in the sclerotome, basal sur face of dermamyotomal cells adjacent to the basal lamina and on scatte red cells throughout the dermamyotome. In further advanced somites in stages 13 and 16, alpha 6 immunoreactivity became restricted to the my otome. alpha 6 was expressed on mesenchymal core cells within the myoc ele of undifferentiated epithelioid somites and the ventromedial wall of somites commencing differentiation at each stage. beta 1 distributi on resembled that of alpha 6 in stage 11 semitic tissue, however, it r emained present on myotome and sclerotome cells in the later stages, a nd was also expressed on dermatomal cells in stage 16. Laminin immunor eactivity, while more intense and prevalent than alpha 6 and beta 1 in each stage examined, occurred on the same somite cell populations as the 2 integrin subunits. These results show a defined distribution of alpha 6 on semitic tissue, and suggest this integrin is involved in so mite differentiation. They also support a possible role for alpha 6 in myoblast formation and migration. Overlapping of beta 1 and laminin i mmunoreactivity with that of alpha 6 further suggests that alpha 6 pai rs with beta 1 as a functional heterodimer for laminin in defined semi tic regions.