CROSS-REACTIONS OF SERA FROM TOXASCARIS-LEONINA AND ASCARIS-SUUM INFECTED MICE WITH TOXOCARA-CANIS, TOXASCARIS-LEONINA AND ASCARIS-SUUM ANTIGENS

The ELISA method using larval ES products and homogenized Toxocara can is, Toxascaris leonina and Ascaris suum adult worms extract, was used to determine the possible cross-reactions in BALB/c and C57BL/10 mice inoculated with embryonated eggs or adult worms extract of T. leonina or A. suum in single and multiple doses. When we used sera of mice inf ected with embryonated eggs of T. leonina against different heterologo us Ag, no cross-reactions against T. canis ES and A. suum ES Ag were o bserved using a single dose. Similarly, in multiple doses no response against T. canis ES Ag was observed. In mice inoculated with adult wor ms extract of T. leonina cross-reactions with T. canis ES and A. suum ES Ag did not occur. Sera from BALB/c mice infected with embryonated e ggs of A. suum, was tested using ES Ag from both A. suum and T. canis and no reactions were observed. This fact confirmed the resistance of this murine strain to A. suum embryonated eggs. When we used sera of s usceptible C57BL/10 mice infected against different heterologous Ag, w e observed no cross-reactions against T. canis ES Ag. In the case of b oth BALB/c and C57BL/10 mice immunized with a single dose of A. suum a dult crude extract no cross-reactions were seen against ES T. canis Ag and with sera from C57BL/10 mice against ES T. leonina. These facts c onfirmed the specificity of the ES T. canis Ag.