H. Vally et al., PURIFIED RAT PERITONEAL MAST-CELLS - CHARACTERISTICS OF THE REMOVAL AND BINDING OF IGE, Immunology and cell biology, 73(3), 1995, pp. 212-219
Mast cells were purified by discontinuous density gradient centrifugat
ion to greater than 90% and subsequently exposed to lactic acid in ord
er to dissociate endogenous IgE. Unfortunately no removal of this IgE
was achieved, despite previous reports of lactic acid exposure dissoci
ating IgE from human basophils. Only a minimal level of endogenous IgE
was found on the surface of mast cells, however, which allowed invest
igations of IgE binding to proceed without the initial removal of this
endogenous IgE. Conditions for binding of normal rat IgE and myeloma
IgE were established while efforts to bind human IgE to purified rat m
ast cells were unsuccessful. Our results suggest that while purified r
at mast cells have the potential to play an important role in the stud
y of the IgE requirement of various stimuli including histamine-releas
ing factor, this role is limited at present.