A FAST TRANSIENT OUTWARD CURRENT IN CULTURED-CELLS FROM HUMAN PULMONARY-ARTERY SMOOTH-MUSCLE

A voltage-dependent transient outward current (I-to) was observed in c ells cultured from human pulmonary artery smooth muscle when K+, but n ot Cs+, was the dominant cation in the pipette solution. In 30% of cel ls investigated using the Cs+ pipette solution, a tetrodotoxin-sensiti ve inward current (I-in) dependent on extracellular Na+ was evoked fro m depolarizations positive to -30 mV. I-in showed voltage-dependent-in activation with a membrane potential at 50% of the evoked current (V-5 0%) of -75.53 +/- 0.81 mV and slope factor potential (V-s) of -10.73 /- 0.01 mV. In the presence of 1 mu M tetrodotoxin, I-to was rapidly e voked by depolarizations positive to -40 mV and decayed with a single exponential time course (tau = 9.9 +/- 1.1 ms, pulse potential = +50 m V). I-to also showed voltage-dependent inactivation with a V-50% of -7 0.9 +/- 2.63 mV and V-s of -7.7 +/- 0.03 mV. I-to was inhibited concen tration dependently by the K+ channel blocker, 4-aminopyridine (4-AP), with a concentration of 4-AP at which I-to was reduced to 50% of cont rol of 36.5 +/- 2.8 mu M. These cells possess voltage-dependent curren ts characteristic of the K+-selective fast transient outward and Na+-s elective inward currents of smooth muscle cells.