Jg. Daly et al., A COLORIMETRIC ASSAY FOR THE QUANTIFICATION OF BROOK TROUT (SALVELINUS-FONTINALIS) LYMPHOCYTE MITOGENESIS, Fish & shellfish immunology, 5(4), 1995, pp. 265-273
A colorimetric assay using the dye MTT (3-[4,5-dimethylthiazol-2-yl]-2
,5 diphenyl tetrazolium bromide) was developed to quantify the prolife
rative responses of break trout (Salvelinus fontinalis) peripheral blo
od and kidney lymphocytes. Mitogenic responses were assayed using the
mitogens LPS, PHA, ConA and formalin-killed Aeromonas salmonicida. Opt
imal mitogenesis occurred only in the presence of homologous plasma. T
he assay proved to be a simple and reproducible alternative to the tra
ditional H-3-thymidine incorporation assay.