DNA-FINGERPRINTING TO DETECT GENETIC-VARIATION IN RICE USING HYPERVARIABLE DNA-SEQUENCES

The suitability of mini- and microsatellite related DNA sequences capa ble of detecting multiple loci was investigated for their ability to g enerate DNA fingerprints in rice. These included R18.1, a cattle-deriv ed probe, the M13 repeat probe, pV47, a human minisatellite probe: and repeats in the Per gene, telomere, chi sequence and 3' hypervariable region of apolipoprotein B. With the R18.1, pV47 and M13 repeat probes , the level of polymorphism was high enough to identify all of the cul tivars and wild rice species used in this study. R18.1, which showed t he highest level of polymorphism, was estimated to identify up to 2.5 x 10(20) genotypes of rice. In a F-2 population of a 'Basmati-370' and 'Taichung-65' cross, loci detected by R18.1 segregated in a Mendelian fashion. DNA fingerprints were somatically stable and the hybridizati on patterns were identical among different plants of the same cultivar . Application of the above molecular genetic markers for identificatio n of rice genotypes is reported here for the first time.