GENOMIC ANALYSIS IN MARITIME PINE (PINUS-PINASTER) - COMPARISON OF 2 RAPD MAPS USING SELFED AND OPEN-POLLINATED SEEDS OF THE SAME INDIVIDUAL

Two genomic maps were constructed for one individual tree of maritime pine, Pinus pinaster Ait., using a common set of 263 RAPD markers (ran dom amplified polymorphic DNA). The RAPD markers were chosen from a la rger number of polymorphic RAPD fragments on the basis of repeatabilit y and inheritance in a three-generation pedigree. The maps were constr ucted from two independent mapping samples of 62 megagametophytes (ln) from a self cross and from an open-pollinated cross. The markers were grouped (LOD greater than or equal to 4; theta less than or equal to 0.25) and assigned to 13 major and 5 minor linkage groups. Two framewo rk maps were constructed using the ordering criterion of interval supp ort greater than or equal to 3. Comparison of the two framework maps s uggested that the locus order was incorrect for 2% of the framework ma rkers. A bootstrap analysis showed that this error rate was representa tive for our data set. The results showed that framework maps construc ted using RAPD markers were repeatable and that differences in locus o rder for maps of different genotypes or species could result from chan ce, The total map distance was 1380 cM, and the map provided coverage of approximately 90% of the genome.