CHARACTERIZATION OF THE SOYBEAN GENE GMENOD40-2

The GmENOD40-2 gene was isolated from a soybean genomic library and th e nucleotide sequence of a 3 kb EcoRI/HindIII fragment was determined. A 1.7 kb fragment of the GmENOD40-2 promoter region was used in a tra nscriptional fusion construct with GUS. In transgenic Vicia nodules co ntaining this construct, beta-glucuronidase activity was detected in t he pericycle of the nodule vascular bundles as well as in the root per icycle at the attachment point of the nodule.