GLUCOSE SENSOR-BASED ON AN AMPEROMETRIC MICROELECTRODE WITH A PHOTOPOLYMERIZABLE ENZYME MEMBRANE

An amperometric glucose sensor based on a photocurable polyacrylamide membrane, in which the enzyme glucose oxidase is entrapped, is reporte d. The enzymatic membrane is deposited on a planar three-microelectrod e cell by casting and by photolithography patterning. The latter techn ique allows us to obtain reproducible and thin membranes. The glucose sensors developed present a sensitivity from 45 to 67 nA mM(-1) glucos e depending on the membrane thickness and the enzyme concentration in a linear range of 4x10(-3)-1 mM. Sensors with a 10-15 mu m membrane th ickness exhibit a short response time of 20-30 s (t(95%)) and a long-t erm stability of three weeks.