2-DIMENSIONAL CRYSTALLIZATION AND PRELIMINARY STRUCTURE-ANALYSIS OF LIGHT-HARVESTING-II (B800-850) COMPLEX FROM THE PURPLE BACTERIUM RHODOVULUM-SULFIDOPHILUM

Authors
MONTOYA G CYRKLAFF M SINNING I
Citation
G. Montoya et al., 2-DIMENSIONAL CRYSTALLIZATION AND PRELIMINARY STRUCTURE-ANALYSIS OF LIGHT-HARVESTING-II (B800-850) COMPLEX FROM THE PURPLE BACTERIUM RHODOVULUM-SULFIDOPHILUM, Journal of Molecular Biology, 250(1), 1995, pp. 1-10
Citations number
31
Categorie Soggetti
Biology
Journal title
Journal of Molecular Biology → ACNP
ISSN journal
00222836
Volume
250
Issue
1
Year of publication
1995
Pages
1 - 10
Database
ISI
SICI code
0022-2836(1995)250:1<1:2CAPSO>2.0.ZU;2-M
Abstract
Light-harvesting complex II (B800/850) from the purple bacterium Rhodo vulum (Rhv.) sulfidophilum has been isolated using a new protocol. It has been shown by analytical ultracentrifugation and native gels to be most likely an octamer. Two-dimensional crystals have been obtained b y microdialysis The plane group is p42(1)2 with a=b=157 Angstrom. The crystals diffract to 18 Angstrom in negative stain. Projection maps sh ow clearly that LHII is organized in ring-like particles with an outer diameter of about 76 Angstrom.