CRYSTALLIZATION AND PRELIMINARY-X-RAY INVESTIGATION OF LIPOXYGENASE-3FROM SOYBEANS

Soybean lipoxygenase-3 has been crystallized by the vapor diffusion me thod in 16-20% polyethylene glycol (average M(r), 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 degrees C, at a protein concentration o f 8-15 mg/mL. The crystals, which diffract to 3-Angstrom spacings, bel ong to the monoclinic space group C2. Cell constants are a = 111.9, b = 136.4, and c = 61.6 Angstrom and beta = 95.7 degrees. The calculated value of Matthews's constant, V-m = 2.48 Angstrom(3)/kDa, is consiste nt with the presence of one molecule of lipoxygenase per crystallograp hic asymmetric unit (Z = 4).