R. Curtis et al., DIFFERENTIAL ROLE OF THE LOW-AFFINITY NEUROTROPHIN RECEPTOR (P75) IN RETROGRADE AXONAL-TRANSPORT OF THE NEUROTROPHINS, Neuron, 14(6), 1995, pp. 1201-1211
The receptor mechanisms mediating the retrograde axonal transport of t
he neurotrophins have been investigated in adult rats. We show that tr
ansport of the TrkB ligands NT-4 and BDNF to peripheral neurons is dep
endent on the low affinity neurotrophin receptor (LNR). Pharmacologica
l manipulation of LNR in vivo using either an anti-LNR antibody or a s
oluble recombinant LNR extracellular domain completely blocked retrogr
ade transport of NT-4 and BDNF to sensory neurons, while having minima
l effects on the transport of NGF in either sensory or sympathetic, ne
urons. Furthermore, in mice with a null mutation of LNR, the transport
of NT-4 and BDNF, but not NGF, was dramatically reduced. These observ
ations demonstrate a selective role for LNR in retrograde transport of
the various neurotrophins from distinct target regions in vivo.