STRUCTURAL DETERMINANTS DEFINING COMMON STEREOSELECTIVITY OF LIPASES TOWARD SECONDARY ALCOHOLS

In this review we summarize some aspects of the enantiopreference of t he lipase from Candida rugosa following structural analysis of complex es of this lipase with two enantiomers of an analog of a tetrahedral i ntermediate in the hydrolysis of simple esters. The analysis of the mo lecular basis of the enantiomeric differentiation suggests that these results can be generalized to a large class of lipases and esterases. We also summarize our experiments on identification of the key regions in the lipases from Geotrichum candidum lipase responsible for differ entiation between fatty acyl chains.