M. Rossetto et al., USE OF RAPD ANALYSIS IN DEVISING CONSERVATION STRATEGIES FOR THE RAREAND ENDANGERED GREVILLEA-SCAPIGERA (PROTEACEAE), Molecular ecology, 4(3), 1995, pp. 321-329
Extensive human impact in south western Australia has resulted in a hi
gh incidence of rarity throughout the highly endemic flora of the regi
on. Grevillea scapigera (Proteaceae) is a typical example, with 27 pla
nts (represented by four extant populations) remaining in the wild. In
order to devise an appropriate strategy for the conservation of this
species, its population genetics were studied using RAPD analysis, whi
ch enabled the discrimination of individual plants and the detection o
f a relatively high amount of variability (V = 0.32) within G. scapige
ra. This variability was found to be evenly distributed within the pla
nts analysed despite the clear distinction between most populations (8
7% of the variability being attributable to single plant difference an
d 13% to population difference). Finally, RAPD analysis was used to se
lect a small group of plants that captured maximum genetic variability
to be used in the recovery program of the species. Because of the low
genetic difference between populations, the mixing of these selected
plants during the recovery process should not create genetic imbalance
s. The methods used in this study provide a useful model for future pr
ojects involving the recovery of rare flora.